The Italian affiliate of l’Incanto, l’Incanto Italia, participates in a research project financed by the European Community

The Italian affiliate of l’Incanto, l’Incanto Italia, participates in a research project financed by the European Community for the identification of active ingredients extracted from the plants which develop in the South of Italy. The research began its works on numerous present plant extracts in Calabria, very interesting bioactive components from the cosmetic, nutritional but also environmental point of view.

Our researcher as well as the researchers of the Universities of Salerno and Reggio Calabria highlighted the high antioxidizing activity of the skin of the red onion of Tropea, usually considered as a waste, by determining exactly its chemical composition constituted mainly by flavonoids which is a fundamental prerequisite for a more sustainable and productive use in cosmetic, pharmaceutical and nutraceutical formulations.

Also, if the quality, the aroma and the taste of the roots of Calabrian licorice are world-famous, the air parts of the licorice usually considered as wastes were analyzed and turn out to be surprisingly a source of bioactive ingredients for the plant itself and for us because of the presence of numerous components belonging to 2 major classes of polyphenols. From an ecological perspective, the flavonoids in the outer leaves part represent a more efficient plant protection against insects.

Given the antimicrobial and antiinflammatory properties of the main components of the air parts of the licorice, it is obvious that these "wastes" represent a source of potential raw material to recover health-promoting ingredients for cosmetic, nutraceutical and food sectors. Further studies are underway to assess their applications.

The results of these first researches were presented to the following Congresses:

Congress World annual of the Italian Latin American Society of Ethnomedicine, Food and Health (SILAE) in Milazzo (Sicily): World Ethnomedicine Summit from 9 to 13 September 2018

https://www.velp.com/euronet/contenuti/image/events/SILAE18_600(1).jpg

And

Chimali -  XII ° Italian Food Chemistry Congress

Camerino (ITALY) 24-27 SEPTEMBER 2018

 

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CHIMALI - XII Italian Food Chemistry Congress
Camerino, September 24-27 2018

 

Let us not forget the principle of l’Ïncantore :

Look for ingredients in Nature to preserve naturally the beauty enchantment.

 

SUMMARIES

 

Tropea Onion skin: from a waste product to new valuable matrix for bioactivity

Rita Celano1, Teresa Docimo2, Anna Lisa Piccinelli1, Luca Campone1,4, Claudio Cannistrà3, Rosa Di Sanzo4, Sonia Carabetta4 Luca Rastrelli1, Mariateresa Russo5

1Department of Pharmacy, University of Salerno; 2L’incanto Sarl, Catanzaro; 3 Hôpital Bichat - Claude-Bernard, Paris, Francia; 4 Food Chemistry, Authentication, Safety and Sensoromic Laboratory – Università Mediterranea di Reggio Calabria

Introduction

The red onion from Tropea is known for its sweetly taste, which is mainly due to the flavonoids and the alk(en)yl cysteine sulphoxides (ACSOs) compounds. The unique red onion envelope usually considered a waste product, represent a valuable source of biomolecules therefore accurate chemical characterization of this matrix is a fundamental prerequisite for a more sustainable and productive use in pharmaceutical, cosmetical and nutraceutical formulations.

Methods

The outer layers of red skin onion bulbs were collected, dried and finely powdered. Exhaustive extraction of dried materials was performed by ultrasound-assisted extraction (UAE) with aqueous ethanol (70% v/v). A UHPLC-HRMS/MS method was developed and then applied to analyze the onion-skin extract.

Results

The chemical composition of onion-skin extract was determined by UHPLC-DAD-HRMS analysis. Metabolite assignments were made comparing UV/Vis, HRMS and MS/MS spectra with reference standards when available, or with chemo-taxonomic data reported in the literature and databases. The main compounds detected in the extract belonging to two major classes of secondary metabolites: anthocyanins and flavonols. The main flavonols found in this onion type were quercetin and quercetin glucosides, isorhamnetin glucosides, kaempferol glucoside, and, among anthocyanins, cyaniding glucosides. This extract has shown a high antioxidant activity. The activity antioxidant was measured by DPPH, ABTS and ORAC assays.

Conclusions

The obtained results have shown that the onion-skins are a promising and cheap source of bioactive compounds with high antioxidant activity. Furthermore, their use as a substitute for synthetic antioxidants, in addition to increasing food security, would contribute greatly to increase their added value.




Unravelling metabolic plasticity of Glycyrrhiza glabra leaves DOP calabrese by chemical profiling

Rita Celano1, Teresa Docimo2, Anna Lisa Piccinelli1, Claudio Cannistrà3, Antonio Massarotto4, Salvatore Fuda5, Luca Rastrelli1, Mariateresa Russo5

1Department of Pharmacy, University of Salerno;

2 L’incanto Sarl, Catanzaro; 3 Hôpital Bichat - Claude-Bernard, Paris, Francia; 4 Naturemed srl, Cosenza; 5 Food Chemistry, Authentication, Safety and Sensoromic Laboratory – Università Mediterranea di Reggio Calabria

Introduction

Quality, aroma and flavor of Calabrian licorice roots are worldwide recognized, although it’s worth noting that aerial part of this plant , usually treated as waste product, represent a surprisingly source of bioactive metabolites. Indeed distribution of metabolites depicts a peculiar biosynthetic and developmental plasticity. In this work we report the chemical profiling of surface and inner leaf content.

Methods

Licorice aerial parts were harvested in pre flowering and flowering time. Leaves, panicles and flowers in flowering periods were collected dried and were ultrasound extracted with EtOH /H2O 70% three times. Vacuum dried samples were reconstituted in MeOH/H20 1:1 for further qualitative analysis by UHPLC system. Exudates from outer licorice leaves was performed by ETOH brief immersion for 10 sec and vacuum dried , samples were analized as above.

Results / Discussion / Conclusion

UHPLC-ESI-HRMS analyses were performed in negative and positive ionization mode to obtain complementary information useful for characterizing the extracts of leaves, exudates, panicles and flowers in flowering periods. Metabolite assignments were made comparing UV/Vis, HRMS and MS/MS spectra with reference standards when available, or with chemo-taxonomic data reported in the literature and databases. The UHPLC-DAD-HRMSn analysis has allowed to identify 46 compounds belonging to two major classes of secondary metabolites: dihydrostilbenes and flavonoids. The dihydrostilbenes have the same central scaffold with different functional groups on the two aromatic rings: hydroxyl, methoxy and prenyl units. The flavonoids are glycosilated derivatives of quercetin, kaempferol, myricetin and naringenin. All extracts showed UHPLC-UV profiles qualitatively comparable except for exudate, which showed dihydrostilbenes exclusively.

Licorice aerial parts are a very promising reservoir of bioactive molecules for plant itself and humans. From an ecological perspective prenilated flavonoids in the outer leaves part represent a more efficient plant protection against insects. These molecules along with inner leaf content of glycosilated flavonoids augment flavonoid bioactivity by increasing membrane affinity and improving the interaction with target proteins.




Chemical profiling of Glycyrrhiza glabra aerial parts, a waste product of PDO

‘‘Licorice of Calabria’’

Rita Celano1, Teresa Docimo2, Anna Lisa Piccinelli1, Claudio Cannistrà3, Antonio Massarotto4, Salvatore Fuda5, Luca Rastrelli1, Mariateresa Russo5

1Department of Pharmacy, University of Salerno; 2 L’incanto Sarl, Catanzaro; 3 Hôpital Bichat Claude Bernard, Paris, Francia; 4 Naturemed srl, Cosenza; 5 Food Chemistry, Authentication, Safety andSensoromic Laboratory – Università Mediterranea di Reggio Calabria

Introduction

Glycyrrhiza glabra aerial parts are a huge amount of discarded material produced during the cultivation and harvest of PDO ‘‘Licorice of Calabria’’ roots. This waste product represents a potential source of functional and health-promoting ingredients. The chemical profiles of exudates, leaves, panicles and flowers were determined by UHPLC-HRMS to estimate their nutraceutical potential.

Methods

G. glabra aerial parts were harvested in pre-flowering and flowering time from three PDO cultivations in Calabria. Exhaustive extraction of dried materials was performed by ultrasound-assisted extraction (UAE) with aqueous ethanol (70% v/v). Exudates were recovered by immersion of leaves in ethanol (10 sec). A UHPLC-HRMS/MS method was developed and then applied to analyze the aerial part extracts.

Results

Negative and positive UHPLC-HRMS analyses, combined with HRMS/MS spectra, chemo-taxonomic data and databases, allowed the full characterization of the metabolites of Calabrian G. glabra leaf exudate, leaf, panicle and flowers. 46 phenolic compounds were identified in aerial parts belonging to two major secondary metabolite classes: dihydrostilbenes and flavonoids. Dihydrostilbenes have the same central scaffold with different functional groups on the aromatic rings (methoxy and prenyl units). Identified flavonoids are flavanones (naringenin an pinocembrin) and their prenylated derivatives, C- and O-glycosilated flavones and flavonols. Dihydrostilbenes, pinocembrin and prenylated flavanones are the most abundant bioactive compounds of aerial parts and they represent the distinctive metabolites of leaf exudates. Instead, glycosylated flavonoids are present in inner leaves and flowers.

Conclusions

UHPLC-HRMS profiling prove that G. glabra aerial parts are a suitable and rich source of bioactive compounds. Their main compounds have well known antimicrobic and antinflammatory properties and it is evident that these wastes represent a potential raw material to recover health-promoting ingredients for food, cosmetic and nutraceutical sectors. Further studies are in progress to evaluate their potential applications.

12th Congress of Morphological Medicine and Anti - Age

L’ ÏNCANTORE participated in the 12th Congress of Morphological Medicine and Anti - Age on January 26th, 2018 at the University Descartes Paris V, the opportunity for him to present Visolea Radiance day Potion to the numerous present practitioners.

Incantore au congrès de Médecine Morphologique et Anti - Age

 

Articles about CICOLEA in Body Language, the International Magazine of the Esthetic and Anti-Aging Medicine n°11 of April May 2017

Articles about CICOLEA in Body Language, the International Magazine of the Esthetic and Anti-Aging Medicine n°11 of April May 2017

CICOLEA has a moisturizing regenerating activity on the healthy skin, on the cutaneous laxity with a real tightening effect, alone or in association after esthetic treatments, physical processing, Fractional Radio Frequency (Legato, Alma Lasers) for example. The International magazine of the Esthetic and Anti-Aging Medicine Body Language n° 11 of April May 2017 publishes 2 articles exposing  the scientific results of a study about the use of Cicolea in the cutaneous laxity and the stretch marks :
“Radiofréquence et cosméceutiques associés” on the page 30
« Fréquence, son et lumière sur la peau » on the page 54

CICOLEA in the magazine "ANFORM Santé et bien être"

CICOLEA is present in the magazine "ANFORM Health and Wellness in the West Indies-Guyana" of May June 2017 distributed in Guadeloupe..

Cicolea in Santé magazine and Top santé

Cicolea will be present on "Santé Magazine" published on 30/04/2015 and on "Top Santé" published on 31/05/2015 .

Cicolea in Santé magazine and Top santé

 

L’ÏNCANTORE was present at the 9th Congress of Morphological and Anti-Aging Medicine on 2015 January 24-25 in Paris

L’ÏNCANTORE was present at the 9th Congress of Morphological and Anti-Aging Medicine on 2015 January 24-25 in Paris with the presentation of preliminary results of a study including the use of CICOLEA cream on skin.

L'incantore au congrès de médecine anti âge à Paris